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These results demonstrated a robust three-column chromatographic process using an optimized affinity capture step and two ion exchange steps to isolate highly purified, infectious CVA21 particles from high-titer microcarrier cell culture harvests controlled at 34C during infection

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Suitable for low to medium pressure, FPLC, or if GST-tag proteins shall be isolated either in particularly short time or in big amounts

Involved research teams Prof Dirk Brenner is the Deputy Head of Research & Strategy at LIHs Department of Infection and Immunity

doi: 10.3390/ijms23094636