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Chromatin immunoprecipitation (CHIP) CHIP assays were carried out strictly in accordance with the manufacturers operational guidelines (Qiagen, Dusseldorf, Germany)

The MTHFR gene has numerous variants with two specifically that have taken center stage in testing and health outcomes

We also conducted an updated systematic literature search for longitudinal studies reporting GMV changes in patients with depression following clinical treatment, covering publications up to June 2025