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MAGI3 deficiency unleashes catenin conformational change to drive metastatic progression and mTOR inhibitor resistance in ccRCC Cell Death & Disease Protocol for purifying biologically active microtubule severing protein UNC 45A from E.coli using GST affinity and spin columns ScienceDirect Separation with Cytiva Products (GST Fusion Proteins) Millipore Glutathione High Capacity Magnetic Agarose Beads, (1:1 suspension in a 30% ethanol solution) 1 ml Iron Protein Purification
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