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Together, these factors affect germination rate, seedling vigor index (SVI), and early growth, determining whether the outcomes are promotive or inhibitory (Hossain et al., 2019)

processing and refining grains result in significant losses, up to 55% for wheat and up to 88% for maize (38)

While such results are inevitable, confirmatory testing usually resolves these issues

Expression, refolding and purification of recombinant pHLA molecules HLA-A*02:01, HLA-A*24:02:01, HLA-A*23:01, HLA-B*14:02 and HLA-C*07:02 constructs for bacterial expression were cloned into pET24a+ plasmids