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For wheat flour (100 mg), the albumin/globulin fraction was extracted in two steps with 1 mL of 67 mM sodium/potassium phosphate pH 7.6, 400 mM sodium chloride

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New England Biolabs, MA, USA), followed by first-strand cDNA synthesis with the NEBNext Ultra II RNA Library Prep Kit for Illumina (New England Biolabs) and NEBNext Multiplex Oligo for Illumina (New England Biolabs) according to the manufacturers protocols

Epidermis-on-a-chip system to develop skin barrier and melanin mimicking model

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