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73 The activity of GPx is also reduced in diabetes due to a decrease in the availability of its cofactor, selenium

Autophosphorylation assay and mass spectrometry Intact protein mass spectrometry was used to monitor transphosphorylation of TcPINK1-D337N by 15 N-TcPINK1-WT, as described previously 10

Cell culture and analysis JEG3 human choriocarcinoma cells JEG3 cells (ATCCHTB36 TM ) were cultured in six-well plates or coverslips in 20% O 2 at 37C in Eagle's Minimum Essential Medium (EMEM) media (ATCC, 30-2003) containing FBS and penicillinstreptomycin (Visent Inc)