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Using a novel and validated fluorescent KRAS Y137W variant amenable to stopped-flow spectroscopy, we characterized the kinetics of KRAS G12C fluorescence changes upon addition of ARS-853 or AMG 510, noting that ARS-853 addition at 5C elicited both a rapid first phase (attributed to binding, yielding a K d of 36.0 0.7 M), and a second, slower pH-dependent phase taken to represent covalent ligation

Abnormal glucose metabolism, especially hyperglycemia, leads to oxidative stress, microvascular damage, vascular tone and endothelial damage, as well as platelet aggregation and embolism (37, 38)

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Then, 1 H, 13 C, Dept 135, and Dept 90 experiments were conducted on the final products with 16, 400, 200, and 200 accumulations, respectively

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