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The column was washed with 6 bed volumes of wash buffer (50 mM sodium phosphate, 300 mM NaCl and 100 mM imidazole, pH 7.4) and the target protein was eluted with 6 bed volumes of elution buffer (50 mM sodium phosphate, 300 mM NaCl and 300 mM imidazole, pH 7.4)

(2) broader dynamic range, evidenced by the larger activity fold change measured in A7r5 cells overexpressing LOXL2 by BHZ labeling (Fig

Lu Y, Gao J, Zhang S, Gu J, Lu H, Xia Y, et al

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It strengthens the liver