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In addition, copper can affect mitogen-activated protein kinase kinase 1 (MEK1) and mitogen-activated protein kinase kinase 2 (MEK2) and enhance their phosphorylation of ERK1 and ERK2 in a dose-dependent manner, further promoting tumor cell proliferation (85)

Gene Therapies Targeting Mitochondrial Dysfunction in Parkinson's Disease Gene therapy entails the treatment of disease via delivery of a transgene that either replaces or corrects a defective gene or is generally supportive of cells in the disease environment (O'Connor and Boulis, 2015)

The remaining buffer was carefully removed and 3flag-tagged proteins were eluted using 90 l of flag elution buffer (20 mM HEPES-KOH (pH 7.9), 150 mM KCl, 1 mM DTT and 1 mg/ml 3flag peptide, Sigma-Aldrich, F4799)

Afterwards, the solution containing the refolded protein was purified via SEC in refolding buffer 2 on the kta Avant or Go system (Cytiva) with a Superdex 75 Increase 10/300 GL or HiLoad 16/600 Superdex 75 pg column (Cytiva) to check for and remove aggregates at a flow rate of 0.5 or 1 mL/min

H.SreevidyaC.LachmansinghJ.MorrisJ.et al