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For future clinical applications, large-scale and long-term studies are necessary to establish more consistent clinical outcomes and validate the actual efficacy of hydrogen therapy

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Nutrients 16 (9), 1397

2.6 Metabolomic sample collection and analysis Oocyte sample collection and metabolomic analysis were performed based on methods from Zhao et al

Figure 7 Cell Apoptosis and DNA Fragmentation At 13 weeks, there were no significant changes in capase-3 and TUNEL positive cell numbers among the three female experimental groups, although cortex capase-3 and TUNEL positive cells in the SE offspring were more than doubled than the SHAM offspring (Figures 8D,H)