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Following centrifugation, the plasma supernatants were carefully aspirated to avoid contamination with the buffy coat or red blood cells and transferred to pre-labeled, sterile microcentrifuge tubes

Multiple mechanisms are involved in this process, including increased epithelial cell apoptosis, abnormal expression of tight junction proteins, altered intestinal permeability, and endoplasmic reticulum stress

[PMC free article: PMC4446235] [PubMed: 25754159] (Among 899 cases of drug induced liver injury enrolled in a US prospective study between 2004 and 2013, herbal and dietary supplements accounted for 146 cases [16%], but none were specifically linked to yohimbe)

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