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Troubleshooting Reference Chart When protocols do not work as expected, systematic troubleshooting helps identify the issue

It also has some surprising effects on both the brain and the environment

One thing you can do is place the gauze over the injection site right after you remove the needle to limit the amount of leakage

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Samples were then homogenized via a Bioruptor bath sonicator (Diagenode, set on high power with 10 cycles of 10 s pulses with a 10 s pause between pulses, at 4C), then centrifuged at 21,500 x g at 4C for 30 min and the supernatant retained