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siRNA transfection mix ( 20 l of 10nM siRNA and 16 l INTERFERin reagent in 250 l 0.2nm filtered seawater) was pipetted directly on the exposed coral and after ~ 2 minutes, 5 ml of 0.2 nm filtered seawater was added to fully cover the nubbin and coral was incubated at ambient temperature for 6 hours

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