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ASP induced cell cycle G1 phase arrest in EC cells The KLE and Ishikawa were treated with 0.01, 0.1, and 1 mg/mL ASP extracts for 36 h, and an analysis of cell cycle prolife showed that ASP extract induced cell cycle G1 phase arrest in both cell lines (Figure 2A)

I dont think this is a problem that we can regulate ourselves out of because were always producing new designer substances, Piatkowski says

Cell confluence regulates claudin-2 expression: possible role for ZO-1 and Rac

After 24 h, treated biofilm supernatant was washed once with 1 PBS followed by addition of 200 L of 1 PBS plus 15 L resazurin 0.05% w/v solution, and incubated for a further 24 h, at 37C, 150 rpm