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The cleared supernatant after centrifugation was applied to glutathione Sepharose 4B (GE Healthcare) resin and incubated for 2 h

L-buthionine sulfoximine (BSO) is a specific and selective inhibitor of -glutamylcysteine synthase and consequently of GSH synthesis [2]

Currently, we investigated the possible strategies of GHK on ICH injury

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