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Int J Biological Macromolecules

Brains were kept it place by gently overlaying them with 1% UltraPure TM low melting point agarose (ThermoFisher #16520100) dissolved in Schneiders medium

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Rubbing in a circular or back-and-forth motion can spread dirt and bacteria around Allow your skin about a minute to dry before you inject If youre using a multi-dose vial, use a swab to wipe the top of the vial before you draw up Injecting into a muscle step-by-step Draw up with a green needle, then swap to a blue for injecting Insert the needle at 90 degrees to your body