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In such an experimental setting, where [MNA + ]/[MNAH TOT ] is constant but the Nernstian term [MNA + ]/[MNAH] is changing, the theoretical curve of the electrode potential versus pH is given by the following function, as [MNAH] can be expressed from equation (9): where K is the protonation constant of MNAH

Finally, we examined the effects of three GSSG-enhancing chemicals on IKK- glutathionylation (IKK--SSG adduct formation) by IP and immunoblotting