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The Ser-tRNASec is next converted into Sec-tRNASec by an enzime called selenocysteine synthase (either directly or via prhosphoseryl intermediated produced by the phosphoseryl-tRNa kinase, using monoselenophosphate as the substrate) [6]

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At 0.5%, suppression begins to fail within 7-14 days

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Reconstituted vials add another problem: every puncture of the septum creates an opportunity for contamination if technique is sloppy

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Biochemistry 52 , 61456150 (2013)

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