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For NNMT focused work, additional attention is often warranted around assay dynamic range, potential quenching or fluorescence interaction if optical readouts are used, and the selection of orthogonal confirmation methods when interpreting pathway level effects

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All samples were processed by aligning sequencing reads to the mm10 mouse reference genome, removing ambient RNA signatures with SoupX 27 , removing low-quality cells and identifying and removing doublets with DoubletFinder 28 (Fig

If you don't mind getting injections, have nutrient absorption issues, and are willing to make the trip to the doctor's office, then B12 injections may be the best option for you