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10.1038/cdd.2015.93 83 HeG.-N.BaoN.-R.WangS.XiM.ZhangT.-H.ChenF.-S

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Characterization of catalytic activity and structure of selenocysteine-containing hGSTZ1c-1c based on site-directed mutagenesis and computational analysis

Thiols Analysis Services Offered by Creative Proteomics Targeted Biothiols Panel (quantitative) : Cys, Cys-Gly, Hcy, -Glu-Cys, GSH/GSSG , CoA-SH, NAC, Cystine, Homocystine Extended Sulfur Metabolites (semi-quant/quant) : Cysteamine, 3-mercaptopropionic acid, 2-mercaptoethanol, penicillamine Volatile Thiols Fingerprinting (targeted GCMS/MS) : 2-furfurylthiol, 4MMP, 3MH, 3MHA, MFT, benzyl mercaptan, ethanethiol Protein Thiol/Disulfide State (mapping) : Differential alkylation and peptide-level LCMS for relative thiol occupancy Redox Metrics : GSH/GSSG, Cys/Cystine, Hcy/Homocystine ratios and Nernst-based redox potential (optional) Stability & Stress Studies : Thiol loss kinetics under process conditions

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