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Aconitase assay The PC-12 cells were seeded onto a 6-well plate at a density of 5 10 5 cells per well and incubated for 24 h at 37 2 C in a 5% CO 2 -humidified incubator

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Vitamin D receptor is expressed at the level of varicose veins Last but not least, we assessed the vitamin D receptor (VDR) expression in the varicose veins by means of immunofluorescence (Fig

Figure 2 In summary, Metabolomics studies have consistently demonstrated that the intestinal metabolite profiles of IBD patients differ significantly from those of healthy controls, including alterations in short-chain fatty acids (SCFAs), bile acids, and tryptophan-derived metabolites, which are repeatedly observed across independent cohorts (177, 178)

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