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Membranes were blocked for 1 h at room temperature in tris buffered saline with tween 20 (TBST) and 5% non-fat dry milk followed by three washes (15 min for each wash) in TBST with mild shaking

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Johnson CD, Abu-Hilal M

Regarding the anti-fibrosis effect of berberine, the autophagy impairment caused by berberine is incapable of eliminating the increased ROS production and contributes to oxidative stress via a feed-forward loop, which accelerates the ubiquitin-mediated degradation of ferritin and the release of iron

It is also unlikely that VIP agonists would be therapeutically beneficial for restoring glandular secretion in CF, since VIP-mediated secretion is dependent upon CFTR [132]