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doi: 10.1038/s41467-022-33116-z 122 ZhaoHZhangWLuYDongYHeZZhenHet al

Identification and characterization of a novel member of the nerve growth factor/brain-derived neurotrophic factor family

The plasmid pYM-N15 served as the template to generate a linear PCR fragment with primers 5-ACAAAAAGCATACAATCAACTATCAACTATTAACTATATCGTAATACACAATGCGTACGCTGCAGGTCGAC-3(ADH2_S1) and 5-TTGCCGTTGGATTCGTAGAAGATAATGGCTTTTTGAGTTTCTGGAATAGACATCGATGAATTCTCTGTCG-3(ADH2_S4 primer)