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SKU: 32168338784
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The membranes were blocked with 5% skim milk (BD Difco, NJ, United States) in PBS containing 0.05% Tween 20 buffer overnight at 4C and incubated with following primary antibodies for 2 h at room temperature: p38 MAPK antibody (dilution 1:1,000

Also, note that DMSO can oxidize sensitive residues like cysteine over time, so you may need to use degassed solvents or add antioxidants if thats a concern

J Cell Mol Med (2021) 25(23):10798813

When you reconstitute with bacteriostatic water (water containing 0.9% benzyl alcohol as preservative), you introduce water and initiate the degradation clock