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For activation, a 50 l cofactor solution containing 0.9 mM glutathione, 0.1 mM hematin, and 0.24 mM TMPD (N, N, N, N-tetramethyl-p-phenylenediamine dihydrochloride) in 0.1 M Tris HCl buffer with a pH of 8.0 was added to the enzyme solution (10 l) and refrigerated on ice for five minutes

So transdermal supplementation can be really great for clients