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Methotrexate-polyglutamate stimulates pro-inflammatory cytokines, such as TNF-, IL-6, and nuclear factor- B (NF-B), initiating inflammatory reactions that damage hepatocytes

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Metformin treatment altered the expression of mitochondria and protein translation pathways We next used bulk RNA sequencing (RNAseq) to profile the whole blood transcriptome of all subjects with low or no detectable viremia

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Quantitative flow cytometric analysis further confirmed this observation, with mean fluorescence intensity at 8 h measuring 1.63-fold and 4.79-fold higher than values recorded at 4-hour and 2-hour intervals, respectively (Fig