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The permeability of the leak pathway can be better assessed ex vivo by measuring the flux of molecules from the luminal to the basolateral side such as EDTA, mannitol, sucrose, inulin and polyethylene glycols (PEG) or dextran molecules of variable sizes (ranging from 4 up to 20 KDa)

Wittwer ED, Liu Z, Warner ND, Schroeder DR, Nadeau AM, Allen AR, et al

Switching concentrations between vials means recalculating every dose, which introduces error risk

doi:10.1073/pnas.1209474109 Attia, P

Find out if drinking coffee can trigger symptoms of this known condition in the Safety section of our updated article about Coffee and Heart Health