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High Nrf2 and ABCC3 expression was also associated with poor prognosis for other treatments including trastuzumab, anthracycline and taxanes

Nature 617 , 139146 (2023)

1.2 L of each primer was added to 10 L SYBR green mix with 1 L of amplified cDNA and 0.4 L MgCl 2 diluted in RNAse-free water (Thermo Fisher Scientific) to provide a total volume of 20 L in each reaction well