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Protein samples were separated by 412% SDS-PAGE, transferred onto PVDF membranes (GE Healthcare, Milan, Italy), and incubated with antibodies targeting and Alpha-Smooth Muscle Actin (catalog n A2547, Sigma-Aldrich)

F (2, 27) = 3.6), ( i ) measurement of the hind paw and fore paw stride length during the Gait test (n Young = 10 mice, n Aged = 13 mice, n Aged+GDF11 = 11 mice

Relative to the most recent administered dose, the median time to onset was 3 days (range: 1-13 days)

The quadruplet therapy may significantly improve long-term outcomes, according to J&J

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