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These evaluations are more clinically relevant as similar tests are also regularly performed in human

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After incubation with horseradish peroxidase (HRP)-conjugated secondary antibodies (Thermo Fisher Scientific, 1:5000), the proteins were visualized using chemiluminescence reagents (29050, Engreen, San Jose, CA, USA) with a Gel Doc EZ Imager (Bio-Rad Laboratories, Hercules, CA, USA) and analyzed using the Image Lab software (Bio-Rad Laboratories)

While cognitive decline is often associated with aging, it's essential to distinguish between regular age-related changes and more serious cognitive disorders