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The other consistent context in the preclinical literature is a metabolic environment the mouse studies pair the compound with diet/energy-balance conditions rather than testing it in isolation

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Adipocyte Biology and Fat-Cell Function : Investigating how AOD 9604 influences fat breakdown (lipolysis) and fat storage (lipogenesis) in isolated fat tissue or cultured fat cells

A serum B 12 concentration within the normal limit, ie, above 148 pmol/L, does not exclude deficiency