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Real-time fluorescence quantitative PCR Tissue blocks were ground into powder in liquid nitrogen, and RNA was extracted from 50 mg of tissue using the Trizol method

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55 A subsequent international study (2011) showed that increased taxation had reduced the rates of smoking in youth, young adults and low socio-economic status individuals, but that there was insufficient evidence to demonstrate effectiveness for heavy/long-term smokers, people with dual diagnoses (individuals who use tobacco products and have mental health and/or non-nicotine substance use disorders) and Indigenous Peoples