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EOPBM employed a pulsed diode laser (Gemini Diode Laser, Azena Medical LLC, USA) with dual wavelengths (810/980 nm), targeting 10 points across the face and anterior neck, including bilateral submandibular and submental regions (Table 3)

CS and GAPDH were used as loading controls

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All calculations were carried out with the B3LYP functional 50,51 , augmented with Grimmes D3 dispersion correction 52,53 , which already proved to be a good choice for chalcogen-containing systems 54,55

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