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doi: 10.1007/978-1-4419-8216-2_7 498 ManianFA

Front Microbiol 7, 947

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[137] found that the sesquiterpene CA-4 in the seed extract of Artemisia argyi exerts anti-inflammatory effects through a dual mechanism: 1) Inhibiting the MAPK pathway (phosphorylation of ERK/JNK), down-regulating the expression of iNOS/COX-2, and blocking the polarization of M1 macrophages

The cells were then incubated with antibodies against arrestin3 or HA (1:1000 dilution) for 1 h at 20 C, followed by washing with PBS and incubation with the secondary antibodies (Alexa 555-conjugated antibodies at 1:500 dilution)