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GTPase activity analysis To measure the GTPase activity of the DRP1 protein using an ATPase/GTPase Activity Assay kit (#MAK113, Sigma-Aldrich, USA), first, prepare a standard curve for phosphate concentration by mixing phosphate standards and purified water according to the instructions

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FIT uses antibodies to detect the globin protein portion of human hemoglobin

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Key Points Vitamin B12 Deficiency and Nerve Damage There are two ways in which B12 deficiency can lead to nerve damage: Myelinopathy : damage to the myelin sheaths (protective layer covering the nerves) Axonal degeneration : destruction and dying off of the nerve fibres and axons The central nerves of the spinal cord can also be affected, as well as the smallest nerve fibres in the peripheral nervous system especially in the hands and feet