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Increased generation of superoxide by angiotensin II in smooth muscle cells from resistance arteries of hypertensive patients: role of phospholipase D-dependent NAD(P)H oxidase-sensitive pathways

Lipid metabolism Although MDSCs predominantly rely on glycolysis and OXPHOS as a bioenergetic source, lipid uptake and catabolism via fatty acid oxidation (FAO) are now recognized as central metabolic programs that enable MDSCs to survive, expand, and exert potent immunosuppressive functions in the TME (72)

The reaction mixture contained 100 L of enzyme extract, 1,900 L of 50 mM phosphate buffer (pH 7.8), 9.9 mM L-methionine, 57 M NBT, 0.0044% riboflavin, and 0.025% Triton X-100

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The diluted bacterial solution was added to 96-well plates at 200 l per well, 3 parallel wells for each sample, and blank reference using BHI medium