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These intermediates are metabolic precursors of amino acids, nucleotides, fatty acids, hemes, and porphyrins, which are essential components for synthesizing cell membranes, DNA, proteins, and other macromolecules ( It is appropriate to mention that similar to glycolysis, the TCA cycle can generate two ATPs from substrate-level phosphorylation, which occurs when the succinic CoA synthetase enzyme catalyzes the reversible reaction of succinyl CoA to succinate (Martnez-Reyes and Chandel, 2020)

Glucose metabolism accounts for ~1% of circulating ketones in states of low-carbohydrate intake because pyruvate predominantly enters the hepatic TCA cycle via carboxylation to oxaloacetate or malate rather than decarboxylation (to acetyl-CoA) (Magnusson et al., 1991

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The generation of ROS can be the result of various events such as free radical generation in the substantia nigra, abnormal metabolism of dopamine, abnormalities in the respiratory chain of the mitochondria, and increased production of Fe 2+ as a result of Fentons reaction

That is why many successful professional vocalists on an 8 show per week schedule will refrain from excessive voice use between performances