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Next, we performed competitive ABPP experiments by treating SK-MEL-103 cells first with either an SCLA compound or dimethylsulfoxide (DMSO), followed by treatment with a paired alkyne-modified clickable probe

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For example, blood cell copper can be temporarily elevated during inflammatory response while liver levels are not

The 4 um thick paraffin sections were then dehydrated, stained with methylene blue, and washed with distilled water, followed by a repeat of dehydrating and washing the sections in order to prepare for a Nissl staining trial