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Supernatant containing proteins were collected for immunoblotting, extracted proteins (2040 g) were separated by SDS-PAGE (615%) gel and then electroblotted onto Polyvinylidene Fluoride (PVDF) membranes (Amersham Hybond-P, GE-Healthcare Life Science, Pittsburgh, PA, USA)

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However, the abrupt onset and probable insulinopenia, demonstrated by the need for insulin, suggest an autoimmune cause, which was corroborated by the autoantibody test results

10.1158/0008-5472.CAN-14-2676 76 LiS.ChenL.JinW.MaX.MaY.DongF.et al (2017)

Cu K, 40 kV30 mA, /2 scanning, angular step 0.02, 3 s accumulation per step) were carried out