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After cellular uptake and before lysosomal degradation, the nanocarrier successfully escaped from the endosome by photothermally induced endosomal disruption of rGO and the proton sponge effect of BPEI

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The electron transfer process between EB and CDs effectively quenched the blue fluorescence, resulting in a fluorescence color change from blue to dark red, followed by enhancement of the EB fluorescence (bright red) upon introducing DNA into the mixture due to the strong intercalation of EB in DNA

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