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In Vitro Skin Irritation Test HaCaT keratinocytes were cultured in DMEM containing 10% fetal bovine serum and 1% penicillin/streptomycin and were maintained under 37 C with humidified 5% CO 2

If you're ready to move from forum speculation to an actual protocol, the place to start is a consultation with Healthspan's clinical team through the Topical Rapamycin+ for Hair program