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Persistent AGERAGE activation can enhance cytokine production and ROS accumulation, creating a microenvironment that favors TGF-1 activation, extracellular matrix deposition, and fibrotic remodeling (145)

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In this method, 5 m skin tissue sections were deparaffinized, rehydrated, washed, and treated with proteinase K for 15 min for unmasking the antigen, then 3% BSA blocking solution was used to eliminate non-specific binding portions from the sections and incubated with anti-STAT3 and anti-Ki67 (1:100 dilutions) primary antibodies overnight at 4