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In certain embodiments, however, such recombinant human antibodies can be subjected to in vitro mutagenesis and thus the amino acid sequences of the V H and V L regions of the recombinant antibodies are sequences that, while derived from and related to human germline V- and V L sequences, may not naturally exist within the human antibody germline repertoire in vivo

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IGFs were first described in 1957 by Salmon and Daughaday [2] when they noted that direct addition of growth hormone (GH) to costal cartilage from hypophysectomized rats in vitro did not significantly stimulate growth (measured by radioactive sulfate uptake)

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Hing B, Braun P, Cordner ZA, Ewald ER, Moody L, McKane M, Willour VL, Tamashiro KL, Potash JB