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Gentle swirling is always the recommended method

This research was also supported by the Electron Microscopy and Comparative Medicine Shared Resources of the Fred Hutch/University of Washington/Seattle Childrens Cancer Consortium (P30 CA015704), and by the University of Washington/Fred Hutch Center for AIDS Research, an NIH-funded program under award number AI027757 which is supported by the following NIH Institutes and Centers: NIAID, NCI, NIMH, NIDA, NICHD, NHLBI, NIA, NIGMS, NIDDK

Cell culture If not stated otherwise, cells were cultivated in DMEM (Thermo Fisher Scientific) supplemented with 10% FBS (Thermo Fisher Scientific) at 37 C with 5% CO 2 in a water saturated steam atmosphere

Subsequently, Western Blot (WB) analysis detected a decrease in total P53 expression but an increase in phosphorylated P53 (p-P53) expression in OGD-induced senescent endothelial cells post FOXO4-DRI treatment (Figures 5B,C)

Refrigeration, light protection, and sealed storage are still required to maintain GHK-Cu stability