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The peptide has to penetrate the outer skin barrier (stratum corneum), which limits how much active compound reaches the deeper dermal layers where collagen and elastin are produced

Cysteine levels in the supernatant were measured by a Cysteine Assay Kit (#MAK255, Sigma) and the cysteine standards

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[DOI] [PubMed] [Google Scholar] 36.Tian X., He W., Yang R., Liu Y

Yet, whether potential protection conferred by isothiocyanates via the Nrf2-dependent pathway is diminished in individuals carrying GST null variants is currently unknown