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These observations have important implications in harnessing the power of HSCs to alleviate aging-associated conditions and improve healthspan

The primary antibodies used included anti-ZO-1 (Abcam, ab307799, 1:5000), anti-Occludin (Abcam, ab216327, 1:5000), anti-ACSL4 (Abcam, ab155282, 1:10000), anti-TFR1 (ABclonal, A5865, 1:2000), anti-SLC7 A11 (Abcam, ab175186, 1:5000), anti-GPX4 (Abcam, ab125066, 1:5000), anti-VDAC2 (Proteintech, 116631-AP, 1:5000), anti-VDAC3 (Proteintech, 552601-AP, 1:5000), -tubulin (Proteintech, 100681-AP, 1:5000), and GAPDH (Proteintech, 104941-AP, 1:20000)

For the record, there are very very few non-animal sources of natural dietary B12 (i..e not fortified cereals, breads etc) which include only a few types of fungi and some seaweeds, to the best of my knowledge

They do not cause air embolism (that risk applies only to intravenous injection of large air volumes)

Structural biology of kainate receptors