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Turn on the water full force and let it run for 2 minutes

Paulose-Ram et al

Filtrates were centrifuged in an Eppendorf 5810 R at 1200 RPM (290 x G) for 8 min, supernatant was aspirated, and the remaining pellet was resuspended in BMDM media

Prior to incorporating the patient in any clinical trial, it is also recommended that researchers or physicians analyze the structure of the BBB to evaluate the ability of a selected drug to pass across the BBB