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It was found that the enzymatic activities of all immobilized samples directly prepared from cell lysates were comparable to those prepared from the conventional immobilization method using purified enzymes

Interestingly, the analysis of the tumors by immunoblot revealed decreased levels of galectin-1 and a subsequent elevated levels of granzyme B in tumors that were treated with the combination of sertraline and irradiation ( p = 0.047, p = 0.016, respectively) (Fig

The protein supernatant was collected by centrifugation at 15,000 rpm at 4 for 10 min and the protein concentrations were measured by bicinchoninic acid (BCA) protein assay kit (Beyotime, China)

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