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Freshly prepared AX in PBS was incubated in the absence or presence of purified HSA purified from serum for 16 h at 37C (final concentrations of HSA and AX in incubation mixture were 3 mg/ml and 2.5 mg/ml, respectively)

Analysis of these 82 metabolites within the C2 pathways from GSEA revealed that 61 pathways were involved (Supplementary Table 4)

Plasmid constructs FLAG-UBR5 (#37188), HA-GSK3 (#14753), HA-GSK3-S9A (#14754), and HA-GSK3-K85A expression plasmids were from Addgene (USA)

They stimulate collagen production, improve skin texture, reduce the appearance of fine lines and wrinkles, and enhance overall skin firmness

Available as HYS060 (60 capsules) and HYS120 (120 capsules)