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High-resolution bisulfite-sequencing of peripheral blood DNA methylation in early-onset and familial risk breast cancer patients

Cancer as a metabolic disease: implications for novel therapeutics

Flora Guerra, University of Salento, Italy Updates Copyright 2021 Li, van Dijk, Meeldijk, Kok, Blommestein, Verbakel, Kotte, Broekhuizen, Lacl, Goldschmeding, Cheng and Bovenschen

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Subsequent desulfurization using our optimized protocol 34 was not successful for the DAM27 ligation product, but an alternative protocol using 2-mercaptoethane sulfonate (MESNa) instead of glutathione resulted in quantitative conversion of cysteine to alanine giving rise to the native DAM27 monobody sequence (Figs